Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT.
Identifieur interne : 003D07 ( Main/Exploration ); précédent : 003D06; suivant : 003D08Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT.
Auteurs : Burtram C. Fielding [Singapour] ; Vithiagaran Gunalan ; Timothy H P. Tan ; Chih-Fong Chou ; Shuo Shen ; Sehaam Khan ; Seng Gee Lim ; Wanjin Hong ; Yee-Joo TanSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Données de séquences moléculaires, Humains, Liaison aux protéines, Mutation, Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (métabolisme), Protéines de transport (génétique), Protéines de transport (métabolisme), Protéines membranaires (métabolisme), Protéines virales (métabolisme), Protéines virales structurales (métabolisme), Séquence d'acides aminés, Techniques de double hybride, Virus du SRAS.
- MESH :
- génétique : Protéines de transport.
- métabolisme : Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines de transport, Protéines membranaires, Protéines virales, Protéines virales structurales.
- Animaux, Cellules Vero, Données de séquences moléculaires, Humains, Liaison aux protéines, Mutation, Séquence d'acides aminés, Techniques de double hybride, Virus du SRAS.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Carrier Proteins (genetics), Carrier Proteins (metabolism), Chlorocebus aethiops, Humans, Membrane Proteins (metabolism), Molecular Sequence Data, Mutation, Protein Binding, SARS Virus, Two-Hybrid System Techniques, Vero Cells, Viral Envelope Proteins (metabolism), Viral Matrix Proteins (metabolism), Viral Proteins (metabolism), Viral Structural Proteins (metabolism).
- MESH :
- chemical , genetics : Carrier Proteins.
- chemical , metabolism : Carrier Proteins, Membrane Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Viral Proteins, Viral Structural Proteins.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Humans, Molecular Sequence Data, Mutation, Protein Binding, SARS Virus, Two-Hybrid System Techniques, Vero Cells.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.
DOI: 10.1016/j.bbrc.2006.03.091
PubMed: 16580632
Affiliations:
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Le document en format XML
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Fielding</name><affiliation wicri:level="1"><nlm:affiliation>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos Building, Singapore 138673, Singapore. bfielding@uwc.ac.za</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos Building, Singapore 138673</wicri:regionArea><wicri:noRegion>Singapore 138673</wicri:noRegion></affiliation></author><author><name sortKey="Gunalan, Vithiagaran" sort="Gunalan, Vithiagaran" uniqKey="Gunalan V" first="Vithiagaran" last="Gunalan">Vithiagaran Gunalan</name></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H P" last="Tan">Timothy H P. 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Fielding</name><affiliation wicri:level="1"><nlm:affiliation>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos Building, Singapore 138673, Singapore. bfielding@uwc.ac.za</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos Building, Singapore 138673</wicri:regionArea><wicri:noRegion>Singapore 138673</wicri:noRegion></affiliation></author><author><name sortKey="Gunalan, Vithiagaran" sort="Gunalan, Vithiagaran" uniqKey="Gunalan V" first="Vithiagaran" last="Gunalan">Vithiagaran Gunalan</name></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H P" last="Tan">Timothy H P. Tan</name></author><author><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name></author><author><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name></author><author><name sortKey="Khan, Sehaam" sort="Khan, Sehaam" uniqKey="Khan S" first="Sehaam" last="Khan">Sehaam Khan</name></author><author><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name></author><author><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Carrier Proteins (genetics)</term><term>Carrier Proteins (metabolism)</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Membrane Proteins (metabolism)</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Protein Binding</term><term>SARS Virus</term><term>Two-Hybrid System Techniques</term><term>Vero Cells</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Matrix Proteins (metabolism)</term><term>Viral Proteins (metabolism)</term><term>Viral Structural Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Liaison aux protéines</term><term>Mutation</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines de la matrice virale (métabolisme)</term><term>Protéines de transport (génétique)</term><term>Protéines de transport (métabolisme)</term><term>Protéines membranaires (métabolisme)</term><term>Protéines virales (métabolisme)</term><term>Protéines virales structurales (métabolisme)</term><term>Séquence d'acides aminés</term><term>Techniques de double hybride</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carrier Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carrier Proteins</term><term>Membrane Proteins</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term><term>Viral Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de transport</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines de transport</term><term>Protéines membranaires</term><term>Protéines virales</term><term>Protéines virales structurales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Protein Binding</term><term>SARS Virus</term><term>Two-Hybrid System Techniques</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Liaison aux protéines</term><term>Mutation</term><term>Séquence d'acides aminés</term><term>Techniques de double hybride</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.</div></front></TEI><affiliations><list><country><li>Singapour</li></country></list><tree><noCountry><name sortKey="Chou, Chih Fong" sort="Chou, Chih Fong" uniqKey="Chou C" first="Chih-Fong" last="Chou">Chih-Fong Chou</name><name sortKey="Gunalan, Vithiagaran" sort="Gunalan, Vithiagaran" uniqKey="Gunalan V" first="Vithiagaran" last="Gunalan">Vithiagaran Gunalan</name><name sortKey="Hong, Wanjin" sort="Hong, Wanjin" uniqKey="Hong W" first="Wanjin" last="Hong">Wanjin Hong</name><name sortKey="Khan, Sehaam" sort="Khan, Sehaam" uniqKey="Khan S" first="Sehaam" last="Khan">Sehaam Khan</name><name sortKey="Lim, Seng Gee" sort="Lim, Seng Gee" uniqKey="Lim S" first="Seng Gee" last="Lim">Seng Gee Lim</name><name sortKey="Shen, Shuo" sort="Shen, Shuo" uniqKey="Shen S" first="Shuo" last="Shen">Shuo Shen</name><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H P" last="Tan">Timothy H P. Tan</name><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name></noCountry><country name="Singapour"><noRegion><name sortKey="Fielding, Burtram C" sort="Fielding, Burtram C" uniqKey="Fielding B" first="Burtram C" last="Fielding">Burtram C. Fielding</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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